A serological survey of antibodies against chicken anemia agent (CAA) in some commercial chicken flocks was conducted by indirect immunofluorescence technique (IIF) using MSBI cells (lymphoblastic cell line from Marek's disease lymphoma) infected with CAA as a source of antigen, CAA antibody could be detected in 103 out of 146 serum samples collected from broiler breeder, layer and day-old broiler flocks with an incidence of 70%. The highest incidence of positive sera was recorded in broiler breeders (78.12-100%) followed by day-old broilers (57.14-83.33%) and layers (54.54-80%). Further detailed investigations are required in order to find out the situation of the disease in different commercial chicken flocks concerning isolation and characterization of CAA in Egypt.