25 males and 25 female baladi rabbits were being used in the present study. All female rabbits were pretreated with hCG and the animals were divided into five equal groups. The first group was ingected with saline and served as control. The remaining four groups were injected with lutlyase estrumate; Voltarin + Lutylase and + Estrumate respectively. The data obtained showed that injection of both types of PGs significantly decreased prolactin level of female rabbits. In male, lutlyase has a potency to decline prolactin. Both types were antagonized by Voltarin pretreatment. Estrumate significantly increased testesterone level while lutylase has no significant effect alone but it significantly increased the level when injected with Voltarine. In females, testosterone levels were not affected by the treatments. Both type significantly decreased progesterone level and it may be attributed to the luteclytic activity of PGs. Voltarine antagonized the effect of both types of prostaglandins. We concluded that both types were differed in alteration of hormonal levels of male and female rabbits, these different actions were obtained also after pretreatment with Voltarin specially on male testosterone level.