A total of 75 fish flesh samples; 25 each of Clarias lazera, Bagrus bayad and Tilapia nilotica were collected during the application of Ametryne as an herbicide for Nelumbo (Nile-Lily) in the River Nile channels. The collected samples were examined for Ametryne residue by using Gas Chromatography. The mean levels of Ametryne residues in Clarias lazera, Bagrus bayad and Tilapia nilotica were 43, 33, 36 ng/gram fish flesh respectively. The level of Ametryne in Clarias lazera is higher as compared with Tilapia nilotica and Bagrus bayad. The source of contamination to fresh water fishes was discussed.