The value of peak II and E/S Fasciola antigens for diagnosis of experimental and natural sheep Fascioliasis were studied by ELISA. The antibody level of experimentally infected sheep with F.gigantica appeared  at 2 weeks postinfection, gradually increased and the highest concentration of antibodies were found 9-13 weeks postinfection with both antigens. Also, both antigens gave 100% sensitivity for diagnosis of naturally infected sheep with fascioliasis. On the other hand, peak II antigen gave 100% specificity, while E/S antigen gave 94.29% specificity.