Ascaridial nematodes are among the well-known parasites infesting fish, birds and mammals. Crude antigen was extracted from Amplicaecum larvae (AL3), Ascaridia galli (adult worm-AG) and Neoascaris vitulorum (adult worm-AV). The charactcrization of these parasitic nematodes revealed relative homologous polypeptide chains (MW 25.5 KDa) common to the three parasitic species, whereas the UV absorbance scan mode wave length (WL 200/550 nm) as related to the absorbance (AB) value zero-2 recorded an indicative frequency curve. The protein concentration in Amplicaecum, Ascaridia and Neoascaris figured up to 5.47 g%, 4.93 g% and 2.20 g%, respectively.