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372613

THE EFFECT OF PREPARED IMMUNOGLOBULINS RICH FRACTIONS AGAINST EXPERIMENTAL ESCHERI- CHIA COLI INFECTION IN CHICKENS

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

The optimal concentration of ammonium sulphate required for precipitation of maximal amounts of chicken immunoglobulin (Ig) was determined. The third precipitate and first supernatant ‘fluid obtained from treatment of pooled chicken serum with 25, 30, 35, 40, 45 and 50% final concentrations of ammonium sulphate were electrophoretically analyzed in poly-acrylamide gels as well as immuno electrophoretically examined against anti-chicken serum prepared in rabbits. 30% ammonium sulphate concentration was optimal for precipitation of total immunoglobulins from chicken serum, 25% for precipitation of IgM and 45% for precipitation of IgY. When serum IgM or IgY-rich fraction preparation was given 24 hours prior to E-coli (serogroup 078: k 80 infection, protected the experimental birds (about 3: times) against mortality and persistence of the inoculated pathogen than infected no pretreated groups, regardless of the route of administration or class or dose o: Ig used. The degree of protection proves to be dose, route and Ig-class dependent.

DOI

10.21608/vmjg.1992.372613

Authors

First Name

M

Last Name

AWAAD

MiddleName

-

Affiliation

National Research Center , Dokki , Egypt Fac. of Vet. Med. ,Cairo University

Email

-

City

-

Orcid

-

First Name

A

Last Name

HEGAZI

MiddleName

-

Affiliation

National Research Center , Dokki , Egypt Fac. of Vet. Med. ,Cairo University

Email

-

City

-

Orcid

-

First Name

LAILA

Last Name

ALI

MiddleName

-

Affiliation

National Research Center , Dokki , Egypt Fac. of Vet. Med. ,Cairo University

Email

-

City

-

Orcid

-

First Name

M

Last Name

KUTKAT

MiddleName

-

Affiliation

National Research Center , Dokki , Egypt Fac. of Vet. Med. ,Cairo University

Email

-

City

-

Orcid

-

Volume

40

Article Issue

3

Related Issue

49632

Issue Date

1992-07-01

Receive Date

2024-08-08

Publish Date

1992-07-01

Page Start

29

Page End

35

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 40_Issue 3_Pages 29-35.pdf

PDF . 2.2MB

Link

https://vmjg.journals.ekb.eg/article_372613.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=372613

Order

372,613

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

THE EFFECT OF PREPARED IMMUNOGLOBULINS RICH FRACTIONS AGAINST EXPERIMENTAL ESCHERI- CHIA COLI INFECTION IN CHICKENS

Details

Type

Article

Created At

23 Dec 2024