The present study was conducted to explore the effects of acrylamide (ACR) and Nigella sativa (N.S) (which was used as a natural protection) on biochemical, hematological and genetic profiles of Albino rats. The obtained data showed that the lowest values of serum enzymatic activties[Asparatate and Alanine amino transferase (AST, ALT); Gamma glutamyl transferase, (GGT) and Lactate dehydrogenase (LDH) were noticed in sera of rats treated with (ACR) fol- lowed by those given (N.S) with (ACR). Furthermore, the glutathione-S-transferase and reduced glutathione (GSH) values were also significanlty reduced in liver and kidneys of both groups treated with (ACR) after one and two weeks. Insignificant increase in activities of all studied enzymes as well as reduced glutathione (GSH) was recorded in rats treated by Nigella sativa Only, On the other hand, the lowest values of packed cell volume (PCV), hoemoglobin (Hb), RBCs, WBCs, acidophils and lymphocytes were observed in (ACR) treated group allover the experimental period, these results nearly reversed in rats treated with Nigella sativa Only. A slight improvement in biochemical and hematological criteria was observed in rats given (ACR) plus (N.S). The obtained results revealed a Significant decrease in the rate of mitosis in (ACR) treated group during the experimental period. The chromosomal structure and number is Significantly altered in both groups treated with Acrylamide. Finally, the microscopic and ultrastructural changes were observed only in the 14^th day of experiment especially in rats treated with (ACR) and (ACR) plus (N.S), Furherly enclusion lesions were more severe in (ACR) treated group than those given (ACR) plus (NS).