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370851

CLONING AND EXPRESSION OF 3CD GENES OF FMDV

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

RT-PCR amplification of VIA (3D) gene and protease (3C) using its primers. Legation of the PCR product with the cloning vector (using pET system). Expression of the re- combinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA.    

DOI

10.21608/vmjg.1999.370851

Authors

First Name

M

Last Name

SHAWKY

MiddleName

-

Affiliation

FMD Department, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

M

Last Name

SABER

MiddleName

-

Affiliation

Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

First Name

A

Last Name

ABDEL RAHMAN

MiddleName

-

Affiliation

FMD Department, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

A

Last Name

EL-SONOUSI

MiddleName

-

Affiliation

FMD Department, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

Volume

47

Article Issue

3

Related Issue

49549

Issue Date

1999-07-01

Receive Date

2024-07-30

Publish Date

1999-07-01

Page Start

289

Page End

294

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 47_Issue 3_Pages 289-294.pdf

PDF . 1.9MB

Link

https://vmjg.journals.ekb.eg/article_370851.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=370851

Order

370,851

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

CLONING AND EXPRESSION OF 3CD GENES OF FMDV

Details

Type

Article

Created At

23 Dec 2024