The study was conducted on 28 suboestrous and 14 anoestrous buffaloes.The suboestrous buffaloes (Experiment I) were divided into three groups; group I (n=10): 25 mg PGF2α intramuscular (i.m), group 2(n=13); 5 mg PGF2α intrauterine (i.u.) and group 3 (n=5) as control. The aneostrous buffaloes (Experiment II) were placed in two groups: group 1 (n=9): progestogen (ear implant) with pregnant mare serum gonadotrophin (PMSG) regimen and group 2 (n=5): as control. Fixed - time inseminations by frozen semen at 72-96 hr after PGF2α treatment and 48-72 hr after implants removal were done. Strong estrous intensity was observed in 69.2% of i.u. PGF2α group followed by 40% in i.m. PGF2α group. After progestogen + PMSG regimen 44.4% anoestrous buffaloes showed a moderate estrous intensity. Overall conception rates of suboestrous buffaloes treated with different routes of PGF2α were 69% and 60% in group 2 and group 1, respectively compared to 20% in the control group. After treatment of anoestrous buffaloes with progestogen + PMSG, the conception rate was 67%. In conclusion, fixed-time insemination following use of prostaglandin (PGF2α) und progestogen with PMSG regimen can be effectively used for treatment of suboestrous and  anoestrous buffaloes. Moreover, the intrauterine route of PGF2α administration appeared to be equally efficient to the intramuscular route to induce fertile oestrous and increase fertility rate in suboestrous buffaloes.