Mycoplasma gallisepticum (MG) is the most Important pathogen of avian
Mycoplasmosis, in the present study One hundred and fifty positive serum samples for
MG plate agglutination test (SPA) were collected /Vhe included WUNples were one dry
Old chicken (36 sample ), broilers (40 sample ), layers (38 sample ), Balady chicken (@4
sample )and turkeys (22 sample). The samples were culltivated ino mycoplasma medium
containing sterile filtrated swine serum to detect MG. All suspectcd colonies were
identified by conventional method, growth inhibition test and PCI
In this studies culture methods detect MG from 35 and 23.35 percent of the
suspected samples and Polymerase chain reaction confirmed the nuclele acid of the MG
using universal and MG specific primers. Cumulatively PCR confirmed MG
positive in (16%) of samples from 150 field samples collected Curing (he study.
Tissue samples from the positive MG serological cases were examined histo
pathologically for Showing lesions in the tissues. The clinical signs and pathologie lesions
were much more significant in the diseased bird by MG.
The pathological changes in upper respiratory (ract and lung showing large masses
of caseous exudate in the air sacs, predominantly in the abdominal ones and Cpy,
Peritonitis was observed in combined infection with CB. Thoracic airsacs. Bilateral
thoracic as well as abdominal air saculitis with caseous exudate were observed in
severely affected combined cases.