In this study, the in-vitro cytotoxic effect and antiviral properties of gold
nanoparticles (AuNPS) against cytopathic strain (NADL) bovine viral diarrhea virus
(BVDV)were evaluated. AuNPSwas previously reported to possess in-vitro antiviral
properties against HIV and multistrains of influenza virus.Citrated AuNPS of 742 nm
were prepared and PEG functionalized. Evaluation of the cytotoxicity of prepared AuNPS
did not show toxic effects to MDBK cells with concentrations of 2 and 4 ppm. The antiviral
activity of AuNPS was evaluated by the inhibition of the cytopathic effect on infected
MDBK cells by means of (MTT) based colorimetric assay and was found that 4 ppm is the
optimum concentration for virus inhibition. Results of the in- vitro antiviral activity and
cytotoxicity showed that prepared nanoparticles has limited in-vitro toxic effect.