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373661

Comparison of two different extenders for cryopreservation of epididymal dog spermatozoa

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

Post-mortem recovery and cryopreservation of viable sperm from the epididymis of genetically valuable animals or endangered species is an important technique for preserving male gametes and thus for maintaining a germ-plasm bank. The extender type used to freeze sperm is important to avoid spermatozoal membrane damage and to preserve semen quality after cryopreservation. The objective of this study was to determine the effects of commercial bovine extender (BullXcell®, IMV, L'Aigle, France) and Tris-citric acid -fructose egg yolk 7% glycerol extender (TCF) on cryopreservation of canine epididymal sperm. The testes of ten adult Egyptian domestic dogs were kept at 5°C in a saline solution and transported to the laboratory within 24 h. Diluted samples were cooled slowly to 5°C over 2 h and equilibrated at that temperature for 2h. Aliquots of samples were loaded into 0.25 ml straws and frozen in liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen. The semen parameters: Post-thaw progressive motility, viability index, membrane integrity (HOST), and acrosomal integrity (sliver nitrate Stain) were evaluated, Results indicated that membrane integrity (HOST) and intact acrosome were statistically higher in BullX, extender. We concluded that BullXcell® is a suitable alternative for the freezing of canine epididymal sperm. 

DOI

10.21608/vmjg.2015.373661

Keywords

Dog, Epididymal sperm, cryopreservation, BullXcell

Authors

First Name

Salama

Last Name

A

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University

Email

-

City

-

Orcid

-

First Name

Ziada

Last Name

M.S.

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Giza

Email

-

City

-

Orcid

-

First Name

Abdou

Last Name

M.S.S.

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University

Email

-

City

-

Orcid

-

Volume

61

Article Issue

3

Related Issue

49415

Issue Date

2015-07-01

Receive Date

2024-08-13

Publish Date

2015-07-01

Page Start

99

Page End

103

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 61_Issue 3_Pages 99-103.pdf

PDF . 2.5MB

Link

https://vmjg.journals.ekb.eg/article_373661.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=373661

Order

373,661

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Comparison of two different extenders for cryopreservation of epididymal dog spermatozoa

Details

Type

Article

Created At

23 Dec 2024