Liver affection is one of the most challenging conditions in canine medicine practice. The diagnostic workup is exhausting and expensive. The current study is designed to investigate selected cases of dogs' hepatic affections based on clinical, clinic-pathologic, ultrasonographic, and laparoscopic findings with special reference to image-guided biopsy. Thirty-nine diseased dogs of different ages, Sexes, and breeds were involved in this study. The most consistent clinical signs were anorexia (29/39), emaciation (27/39), abdominal distention caused by hepatomegaly (5/39), ascites (19/39), vomiting (18/39), diarrhea (24/39), jaundice (5/39), and fever (2/39). Marked significant Increases in liver enzyme activities (AST, ALT, ALP, and GGT), along with marked elevation in Bilirubin values were the most significant clinicopathological alterations. Seven vaccinated apparently normal dogs from the same locality were used as control, Ultrasonographic assessment was performed on thirty-three patients, the ultrasonographic examination showed fatty changes in liver tissue (11/33), hepatic masses (7/33), cirrhosis (12/33) and biliary tract affections (3/33). Laparoscopy was performed in six patients only. Sub-hepatic abscess and enlarged yellowish liver were observed during laparoscopic procedures, in one case tiny abscess in the ventral surface of the liver along with an enlarged slightly dark-colored gallbladder wall, which was suspected as hepatic Lipidosis, and the diagnosis was confirmed histopathologically. The findings of this investigation assert the importance of ultrasonography and laparoscopy as investigative tools.