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369385

MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

Three field infectious bronchitis virus (IBV) strains isolated from different governorates in Egypt were examined molecularly using reverse transcriptase polymerase chain reaction (RT- PCR) and S1 partial gene sequence. RT-PCR using universal primers was positive for the three isolates. S-1 sequence analysis was successfully performed with two isolates only and failed with the third one. SI analysis of the two isolates revealed that one isolate was identical (100% aminoacid and nucleotide similarities) to Egypt/BeniSeuf/01 of a newly discovered genotype. The other isolate showed close similarity to Dutch variants D274 (98% aminoacid similarity and 99.4 %nucleotide similarity) and D3896 (94% aminoacid similarity and 97.8 %nucleotide similarity). Both isolates showed considerable high divergence to H120 (29.8% amino acid divergence and 25.8% nucleotide divergence for isolate 1 and 26.9% amino acid divergence and 23.9% nucleotide divergence for isolate 2) and M41 (30.4% amino acid divergence and 25% nucleotide divergence for isolate 1 and 28.0% amino acid divergence and 22.6% nucleotide divergence for isolate 2) vaccines.

DOI

10.21608/vmjg.2002.369385

Authors

First Name

H

Last Name

MADBOULY

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Cairo University, Beni-Seuf Branch, Egypt.

Email

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City

-

Orcid

-

First Name

A

Last Name

ABDEL-MONEIM

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Cairo University, Beni-Seuf Branch, Egypt.

Email

-

City

-

Orcid

-

First Name

J

Last Name

JR

MiddleName

-

Affiliation

Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717

Email

-

City

-

Orcid

-

First Name

B

Last Name

LADMAN

MiddleName

-

Affiliation

Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717

Email

-

City

-

Orcid

-

First Name

NANCY

Last Name

ROFAEL

MiddleName

-

Affiliation

Serum and Vaccine Research Institute, Abbasia, Egypt

Email

-

City

-

Orcid

-

Volume

50

Article Issue

4

Related Issue

49349

Issue Date

2002-10-01

Receive Date

2024-07-26

Publish Date

2002-10-01

Page Start

1,053

Page End

1,064

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 4_Pages 1053-1064.pdf

PDF . 3.3MB

Link

https://vmjg.journals.ekb.eg/article_369385.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369385

Order

369,385

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES

Details

Type

Article

Created At

23 Dec 2024