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369375

EFFICACY OF VACCINATION STRATEGY AGAINST LUMPY SKIN DISEASE WITH THE USE OF NEW GENERATION OF INACTIVATED VACCINE

Article

Last updated: 03 Jan 2025

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Tags

Virology and viral diseases

Abstract

Lumpy skin disease (LSD) local strain (Ismalia 88) was propagated in MDBK cell line for six serial passages to obtain a titer of 107.5 /ml TCID50. Harvestation of the adapted virus from inoculated cell culture was based on the time at which a clear CPE was observed (5 days post inoculation). The virus was inactivated using binary ethyleneimine (BEI) at concentration of 2% for 12 hours. The inactivated virus was used with new generation of adjuvant (Nigella sativa oil) in the preparation of inactivated LSD local vaccine. The prepared vaccine was characterized using different techniques including the drop test, emulsion viscosity and stability. In addition, it was tested for the sterility and safety. Twenty Friesian calves (7 month-old) divided into 4 groups were inoculated with the different vaccination proGrammes as follow: group I was vaccinated with 2 doses of sheep pox vaccine with 6 months interval; group 2 was vaccinated with sheep pox vaccine and boostered after 6 months by the prepared LSD inactivated vaccine; group 3 was vaccinated with 2 doses of prepared inactivated LSD vaccine with 6 months interval and group 4 was left non vaccinated calves as negative control. Sera were collected monthly post vaccination for 8 months. The sera were tested by virus neutralization test and ELISA to evaluate the immune response of different vaccination strategies. The results of the in Vivo experiment revealed that the inactivated LSDV adjuvated with Nigella sativa oil was sterile, safe and elicited hight antibody response against LSD either when used for priming or boostering of animals alone or in combination with sheep pox vaccine.

DOI

10.21608/vmjg.2002.369375

Authors

First Name

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Last Name

MADBOULY

MiddleName

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Affiliation

Department of Virology, Faculty of Veterinary Medicine, BeniSuef Branch, Cairo University

Email

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City

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Orcid

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First Name

H

Last Name

HUSSEIN

MiddleName

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Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt

Email

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City

-

Orcid

-

First Name

FATEHIA

Last Name

MOHAMED

MiddleName

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Affiliation

Department of Virology, Animal Health Research institute, Dokki Giza-Egypt.

Email

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City

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Orcid

-

First Name

OMYMA

Last Name

EL-DESAWY

MiddleName

-

Affiliation

Department of Virology, Animal Health Research institute, Dokki Giza-Egypt

Email

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City

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Orcid

-

First Name

M

Last Name

SABER

MiddleName

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Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt

Email

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City

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Orcid

-

First Name

M

Last Name

SHALABY

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt

Email

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City

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Orcid

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Volume

50

Article Issue

4

Related Issue

49349

Issue Date

2002-10-01

Receive Date

2024-07-26

Publish Date

2002-10-01

Page Start

963

Page End

975

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 4_Pages 963-975.pdf

PDF . 4.0MB

Link

https://vmjg.journals.ekb.eg/article_369375.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369375

Order

369,375

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

EFFICACY OF VACCINATION STRATEGY AGAINST LUMPY SKIN DISEASE WITH THE USE OF NEW GENERATION OF INACTIVATED VACCINE

Details

Type

Article

Created At

23 Dec 2024