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369372

PATHOGENESIS OF LOCAL ISOLATE OF RABBIT HAEMORRHAGIC DISEASE VIRUS (RHDV) IN BALADY RABBITS AND CELL CULTURE

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Immunology / vaccine / diagnosis

Abstract

The Balady rabbif) were I/N experimentally inFected with RHDV to study the pathogenesis of The virus. The virus induced 100% morbidity and mortality. The virus antigen(s) were detected in Liver, spleen, lung, heart and kidney by HA, AGPT AND FAT. The virus amounts were inCreased gradually after infection and reached 13 Log, HA titer after 48 hours in liver tissues folLowed by spleen and lung 9 log,, heart tissues 8 Log, and finally kidney tissues 6 log, HA titer. The virus take about 5 days to express its cytoPathic effect (CPE) on primary rabbit kidney cells In the first passage and decreased to 2 days in the Fourth passage in which infectivity titer of the viRus increased 2.8 logo TCIDs0/ml than he first Passage. In case of isolation on Vero cells, the viRus take about 7 days to appear its CPE in the first Passage and decreased to 3 days in the fourth pasSage. The infectivity titer of the virus increased 3.5 logo TCID50/ml in the fourth passage than in The first passage. The haemagglutinin was loss Completely after the third passage in both cell culTure used. This study reflected that the preparation Of RHDV vaccine using cell culture is not preFerred due to the losses of HA antigen during Propagation.

DOI

10.21608/vmjg.2002.369372

Authors

First Name

H

Last Name

MADBOULY

MiddleName

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Affiliation

Faculty Vet. Medicine, Beni-Suef, Cairo University.

Email

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City

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Orcid

-

First Name

M

Last Name

KHODEIR

MiddleName

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Affiliation

Serum and Vaccine Research Institute, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

A

Last Name

MOSTAFA

MiddleName

-

Affiliation

General Organization for Veterinary Services

Email

-

City

-

Orcid

-

Volume

50

Article Issue

4

Related Issue

49349

Issue Date

2002-10-01

Receive Date

2024-07-26

Publish Date

2002-10-01

Page Start

955

Page End

962

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 4_Pages 955-962.pdf

PDF . 2.1MB

Link

https://vmjg.journals.ekb.eg/article_369372.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369372

Order

369,372

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

PATHOGENESIS OF LOCAL ISOLATE OF RABBIT HAEMORRHAGIC DISEASE VIRUS (RHDV) IN BALADY RABBITS AND CELL CULTURE

Details

Type

Article

Created At

23 Dec 2024