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369362

RELATIONSHIP OF BIOCHEMICAL CONSTITUTES OF FOLLICULAR FLUID, HISTOPATHOLOGICAL CHARACTERISTICS OF OVARIES AND IN VITRO MATURATION OF OOCYTES IN BUFFALO COWS DURING DIFFERENT STAG

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Last updated: 03 Jan 2025

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Biochemistry

Abstract

Forty six of buffalo ovaries were collected from slaughter houses. Predicting stages of estrous cycle Were identifiable according to the cyclic changes of the corpus luteum (Stage I, day 1-4; stage II, day 5-10; stage III, day 11-17; stage IV, day 18-21 of the estrous cycle). Oocytes recovered from different stages were classified according to their, as good quality (compact and partially com- pact cumulus), and poor quality (expanded, denuded and degenerated) oocytes. Good quality oocytes were in-vitro cultured to study the influence of different stages of estrous cycle on in-vitro maturation of oocytes. Histopathological studies of right and left ovaries from different stages were performed to investigate the growth and atresia of follicles that aid in understanding the complexity of events associated with maturation of buffalo follicle and oocytes. Moreover, follicular fluids were collected and analyzed for intra-follicular Hormonal and biochemical constituents. Stage III provided the highest rate of good quality oocytes that resumed meiosis to metaphase II. Histopathological findings revealed atrestic follicles and degeneration of granulose cells at different stages of the estrous cycle. Follicular progesterone and estradiol levels and their ratio (P: E) were significantly peaked (P < 0.01) during stage III (day 11-17), while testosterone started to in- crease at stage IV and reached its maximum at stage I of the cycle. Total protein, albumin, globulin, triglycerides, sodium, potassium, calcium and inorganic phosphorus were significantly increased (P < 0.01) in follicular fluid during stage III. On the other hand, the lowest levels of total lipids. Cholesterol, creatinine, urea and transaminase enzyme (AST & ALT) were observed at stage III.

DOI

10.21608/vmjg.2002.369362

Authors

First Name

H

Last Name

ABBAS

MiddleName

-

Affiliation

Hormone Lab., Biochemical Department, Animal Health Research Institute.

Email

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City

-

Orcid

-

First Name

R

Last Name

YOUSSEF

MiddleName

-

Affiliation

Hormone Lab., Biochemical Department, Animal Health Research Institute.

Email

-

City

-

Orcid

-

First Name

NARIMAN

Last Name

RAHMY

MiddleName

-

Affiliation

Clinical Pathology Unit, Pathology Department, Animal Health Research Institute.

Email

-

City

-

Orcid

-

Volume

50

Article Issue

4

Related Issue

49349

Issue Date

2002-10-01

Receive Date

2024-07-26

Publish Date

2002-10-01

Page Start

875

Page End

894

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 4_Pages 875-894.pdf

PDF . 6.9MB

Link

https://vmjg.journals.ekb.eg/article_369362.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369362

Order

369,362

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

RELATIONSHIP OF BIOCHEMICAL CONSTITUTES OF FOLLICULAR FLUID, HISTOPATHOLOGICAL CHARACTERISTICS OF OVARIES AND IN VITRO MATURATION OF OOCYTES IN BUFFALO COWS DURING DIFFERENT STAG

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Article

Created At

23 Dec 2024