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369260

BIOLOGICAL AND IMMUNOLOGICAL CHARACTERIZATION OF ESCHERICHIA COLI PILI ISOLATED FROM CASES OF URINARY TRACT INFECTION IN HORSES

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Bacteriology and bacterial diseases

Abstract

The bacteriological examination of 126 urine samples from horses with urinary tract infection revealed the isolation of E. coli from 76 cases (60.3%); Streptococcus spp. 14 cases (11.1%); Klebsiella spp. 12 cases (9.5%); Proteus spp. 11 cases (8.7%); Staphylosoccus spp. 7 cases (5.6%) Corynebacterium 3 cases (2.3%) and 3 cases were bacteriologically negative (2.3%). The incidence of E. coli isolation from mares was higher (63.9%) than stallions (55.6%). Three E. coli serogroups were identified 0:16, (26 isolates 20.6%), 0:2, (20 isolates 15.9%), and 0:6 (16 isolates 12.7%). The SDS-PAGE analysis of the pur- ified pili preparations from each serogroup revealed one distinct protein band 20.1kDa for 0:16 and 0:6, whereas a 16.6kDa band serogroup for the serogroup 0:2. The hemagglutintion activity of E. coli isolates to the different erythrocyte types was similar to their corresponding purified pili preparations. The effect of D-mannose on the hemagglutination of different erythrocytes revealed two types of hem- agglutination, the mannose sensitive (pili type I) and the mannose resistant hemagglutination (pili type II). In the in-vitro adherence test of E. coli isolates to urinary tract epithelial cells (UTEC), all E. coli isolates were highly adherent, especially the isolates of the serogroup 0:16. In the adhesion inhibition test using the homologus antipili antisera. The number of E. coli adhered to UTEC was significantly (P<0.01) reduce, whereas the hetrologus antibodies could reduce the adhesion in case of isolates of the serogroup 0:16 and 0:6 only. While heterologus antipili antisera could not inhibit the adhesion of the isolates from the

DOI

10.21608/vmjg.2002.369260

Authors

First Name

ANSAM

Last Name

SALMAN

MiddleName

-

Affiliation

Bacteriology Department, Animal Health Research Institute, Doki, Giza

Email

-

City

-

Orcid

-

First Name

R

Last Name

SELEIM

MiddleName

-

Affiliation

Bacteriology Department, Animal Health Research Institute, Doki, Giza

Email

-

City

-

Orcid

-

First Name

SAHAR

Last Name

SAHAR

MiddleName

-

Affiliation

Bacteriology Department, Animal Health Research Institute, Doki, Giza

Email

-

City

-

Orcid

-

First Name

HODA

Last Name

ZAKI

MiddleName

-

Affiliation

Bacteriology Department, Animal Health Research Institute, Doki, Giza

Email

-

City

-

Orcid

-

Volume

50

Article Issue

3

Related Issue

49348

Issue Date

2002-07-01

Receive Date

2024-07-25

Publish Date

2002-07-01

Page Start

377

Page End

388

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 3_Pages 377-388.pdf

PDF . 3.2MB

Link

https://vmjg.journals.ekb.eg/article_369260.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369260

Order

369,260

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

BIOLOGICAL AND IMMUNOLOGICAL CHARACTERIZATION OF ESCHERICHIA COLI PILI ISOLATED FROM CASES OF URINARY TRACT INFECTION IN HORSES

Details

Type

Article

Created At

23 Dec 2024