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369246

RECENT TECHNIQUE TO DETERMINE VIABILITY OF YXOSPOREAN SPORES

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Fish / aquaculture

Abstract

A vital staining technique with fluorescein diacetate (FDA) and propidium iodide (PI) was used for determining the viability of myxosporcan stage spores of Myxobolus species. Examined spores were collected in different ways from live infected tilapia fish, either naturally released from infected fish or collected from pseudocyst of infected fish. Separated spores from infected fish meal were collected and examined. In vitro survivability tests namely drying, aging, freezing and heating were done on collected spores and their viability under fluorescent microscopy was evaluated, viable spores stained green with FDA while non-viable stained red with Pl. Sporicidal testing of some chemicals revealed that calcium hydroxide was the most effective among other sporicidal treatments. Formalin and sodium chloride had a moderate effect on discharged spore viability and isolated spores were relatively sensitive. Vital Staining by FDA-PI can examine efficiency of manufacturing processes of infected fish meal. The results proved that pasteurization of fish meal is the only accurate preventive method.

DOI

10.21608/vmjg.2002.369246

Authors

First Name

JAHLA

Last Name

EL-KHATIB

MiddleName

-

Affiliation

Fish Diseases Department, Animal Health Research Institute, Dokki, Giza, Egypt.

Email

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City

-

Orcid

-

Volume

50

Article Issue

2

Related Issue

49347

Issue Date

2002-04-01

Receive Date

2024-07-25

Publish Date

2002-04-01

Page Start

195

Page End

202

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 50_Issue 2_Pages 195-202.pdf

PDF . 3.0MB

Link

https://vmjg.journals.ekb.eg/article_369246.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=369246

Order

369,246

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

RECENT TECHNIQUE TO DETERMINE VIABILITY OF YXOSPOREAN SPORES

Details

Type

Article

Created At

23 Dec 2024