Out of 100 samples taken from the surface of the skin of poultry carcasses in poultry slaughter- houses, 54 samples were positive to Salmonella enterica serovar enteritidis with a percentage of (54%), 100 fecal samples were taken from human stools of workers in contact with poultry in poultry slaughterhouses at Kalyobia Governorat, suf- fering from diarrhaea and/or fever. Salmonella enterica serovar enteritidis represented in 42 samples with percentage of (42%). Phage typing of isolated strains from poultry and poultry attendant demonstrated three strains 6, 21 and 28 having the possibility of cross infection between poultry men and poultry carcasses. Antimicrobial sensitivity test proved those Salmonella enterica isolated strains were sensitive to Ampicillin (10 µg). Amoxicillin (20 µg), Gentamycin (10 µg), Kanamycin (30 µg), Nitrofurantoin (300 µg), and Cephalothin (30 µg) and medium resistant to Streptomycin (10 µg), and Tetracycline (30 µg). The public health significance of the isolated strains was discussed.