The present study was performed on Forty three bulk tank milk samples collected from different private dairy farms. The samples were cultivated on the selective media, broth and agar plates of Listeria species. Listeria species could be isolated from the examined samples (17) isolates; these were represented by a percentage of (39.5%). Listeria species included (L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri, L. grayi, and L. murrayi). While the detection and isolation of Listeria monocytogenes (6) isolates, that represented (13.95%). The identification of L. monocytogenes was based on the use of the morphological, biochemical, pathological and serological characters.