The aim of this study is to determine the prevalence of pathogenic Salmonella isolates recovered from poultry farms in Egypt. A total of 36 (29%) Salmonella isolates were recovered from 124 samples of poultry (broilers, layers, and breeders) from different governorates in Egypt. The Salmonella isolates were confirmed by invA gene detection. The molecular characterization was done by using spvA gene to identify Salmonella Enteritidisand fliC gene to identify Salmonella Typhimurium by PCR. The 36 isolates (29%, 36/124) were genotyped by sequence analysis of invA gene PCR product. The sequence analysis revealed that, the isolates belonged to three different serotypes, including S. Heidelberg (n = 17), S. Gallinarum (n = 6), and S. Newport (n = 1). Whereas spvA gene was used for detection of S. Entritidis (n = 8).