This study was performed to compare between healthy and equine metabolic syndrome (EMS) regarding serum activity of GGT, CK, LDH, triglycerides, total protein, albumin, globulin concentrations as well as fasting insulin and fasting glucose levels.12 ponies (control, n=5 and EMS cases, n=7) were examined physically and biochemically at private sector in Giza Government. Combined glucose insulin test was performed to estimate insulin sensitivity in EM5 ponies. Ponies of 7 - 9 body condition score (BCS) suffered from EMS showed significant increase in serum GGT activity (P<0.01), triglycerides (P<0.01), fasting glucose (P<0.05) and fasting insulin level (P<0.05) with no significant increase in serum CK activity and decrease in serum LDH activity. The application of CGIT showed significant increase (P<0.001) in serum insulin and glucose levels at the minute 45 when compared with zero time which confirmed the insulin resistance. The obtained data can be used in the diagnosis of EMS cases.