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368303

The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model

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Last updated: 03 Jan 2025

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Tags

Surgery, anesthesia, diagnostic imaging

Abstract

 This study was carried out to verify the clinical efficiency of amphotericin B to induce arthritic changes when injected intra-articularly in equines and to correlate to the histopathological findings. Twenty mg Amphotericin B were injected in right radio- carpal joints of 9 donkeys. Animals were grouped equally into three groups based on the observation time period after injection-(3, 6 and 9 weeks) to evaluate the different degrees of arthritis, Clinical, radiological, histopathological assessments together with synovial fluid analysis were done.        The arthritic changes were observed in all groups. Acute inflammatory reaction developed few hours post injection manifested by lameness, swelling of the joint with significant increase in total protein, enzymes, cell count and mucin precipitation. Macroscopic findings were limited to discoloration of the articular cartilage, few hemorrhagic areas in the joint capsule with slight roughness of the articular surface in old cases. Radiographic arthritic evidences included synovial capsuler swelling in acute cases and narrowing of the joint space in chronic ones. Histopathological changes revealed degeneration and thinning of both cartilage and bone, synovitis, capsulitis and osteophytes formation. The cartilage matrix lost its architecture with fibrous replacement. In conclusion: different degrees of persistent arthritis could be achieved with a single IA injection of amphotericin B in Equine. Joints of these experimental animals are considered as a model of arthritis and can be used for testing different recent treatment techniques.

DOI

10.21608/vmjg.2011.368303

Keywords

Amphotericin B, Arthritis, equine, model, synovial analysis

Authors

First Name

Shaman

Last Name

A

MiddleName

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Affiliation

Surgery, Anesthesiology and Radiology Dept., Faculty of Veterinary Medicine. Cairo Univ., Egypt.

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Orcid

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First Name

Mokbel

Last Name

A

MiddleName

-

Affiliation

Rheumatology and Rehabilitation, Faculty of Medicine. Cairo Univ., Egypt.

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City

-

Orcid

-

First Name

El-Tookhy

Last Name

O

MiddleName

-

Affiliation

Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo Univ., Egypt

Email

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City

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Orcid

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First Name

Mostafa

Last Name

A

MiddleName

-

Affiliation

Pathology, National Cancer Institute. Cairo Univ., Egypt.

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-

City

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Orcid

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Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

397

Page End

415

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 397-415.pdf

PDF . 3.4MB

Link

https://vmjg.journals.ekb.eg/article_368303.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368303

Order

368,303

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model

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Type

Article

Created At

23 Dec 2024