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368298

Detection of Gastric Helicobacter like Organisms (GHLO) in Pet Animals by culture, ELISA and rapid urease test

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Bacteriology and bacterial diseases

Abstract

 A total number of 50 stool, 50 saliva and 50 serum samples were randomly collected from 50 diseased dogs and cats of different breeds hosted in pet animals hospital in port- Said governorate. In addition 23 stomach samples were collected from domestic cats recently euthanized. Samples were prepared and plated on Brain heart infusion agar containing 5% horse serum and Dent supplement antibiotic and selective media. Colombia agar with 7% sheep blood and Dent supplement antibiotic. Each type of specimens. were subjected to isolation by culture and identification by biochemical activity and ELISA. The results demonstrate that among 50 studied dogs, GHLO isolates from stool and saliva samples were found to be 13 (26%) and 18 (36%) respectively and 31 out of 50 (62%) were +ve by ELISA. Also the results demonstrate that among 50 studied cats, GHLO isolates from stool and saliva samples were found to be 10 (20%) and 11 (22%) respectively and 25 out of 50 (50%) were +ve by ELISA. Selective 23 studied cat's stomachs, the results revealed that GHLO isolates were found to be 30.4% were +ve using culture and 47.8% and 52.2% were +ve using rapid urease test (RUT) and ELISA, respectively

DOI

10.21608/vmjg.2011.368298

Authors

First Name

El-S. Enany

Last Name

M

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Khafagy

Last Name

A

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Ibrahem

Last Name

H

MiddleName

-

Affiliation

Microbiology Department, Faculty of Medicine, Jazan University

Email

-

City

-

Orcid

-

Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

355

Page End

362

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 355-362.pdf

PDF . 1.3MB

Link

https://vmjg.journals.ekb.eg/article_368298.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368298

Order

368,298

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Detection of Gastric Helicobacter like Organisms (GHLO) in Pet Animals by culture, ELISA and rapid urease test

Details

Type

Article

Created At

23 Dec 2024