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368248

Sero-diagnostic efficacy of antigens prepared from B. abortus and B. melitensis used for agglutination tests and IELISA

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

 In this study, 120 bovine and ovine serum samples were collected and tested for brucellosis with Rose Bengal test (RBT), modified Rose Bengal test (mRBT), buffered acidified plate test (BAPT) using conventional Rose Bengal antigen (RBA) and buffered acidified plate antigen (BAPA) and antigens prepared from B. melitensis (the main cause of brucllosis in Egypt) strain 16- M. Indirect ELISA was used for testing the same serum samples using 4 different coating antigens which were S-LPS and OMP antigens prepared from 16-M strain and S- LPS and OMP prepared from S19 strain. There were some differences between the results of conventional Rose Bengal and BAPA antigens and Rose Bengal and BAPA antigens prepared from B. melitensis 16-M strain. In the same time, there were no characteristic differences in results of Indirect ELISA  

DOI

10.21608/vmjg.2011.368248

Authors

First Name

Mehanna

Last Name

A

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo (VSVRI)

Email

-

City

-

Orcid

-

First Name

Sadek

Last Name

A

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo (VSVRI)

Email

-

City

-

Orcid

-

First Name

Shell

Last Name

A

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo (CLEVB)

Email

-

City

-

Orcid

-

Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

219

Page End

233

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 219-233.pdf

PDF . 5.4MB

Link

https://vmjg.journals.ekb.eg/article_368248.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368248

Order

368,248

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Sero-diagnostic efficacy of antigens prepared from B. abortus and B. melitensis used for agglutination tests and IELISA

Details

Type

Article

Created At

23 Dec 2024