Subjects
-Tags
Immunology / vaccine / diagnosis
Abstract
Hemolysin (amboceptor) which is the most important constituent in complement fixation test (CFT) was prepared by inoculation of sheep RBCs S/C in Bosket rabbits in two concentrations (10% and 20%)
Blood samples were obtained weekly from inoculated rabbits to measure the titre of induced Hemolysin by CFT. It was found that Hemolysin titer was increased with the number of inoculation till the 7th inoculation then began to decrease with the 8th inoculation. However the titer of Hemolysin obtained from rabbits inoculated with 20% sheep RBCs was higher (1/5800) than that obtained from rabbits inoculated with 10% red cell suspension (1/4500). Similar results were reported to the imported Hemolysin. By using the prepared Hemolysin in CFT for diagnosis of FMD and Brucellosis it gave the same results as that obtained by imported one
DOI
10.21608/vmjg.2011.368247
Authors
MiddleName
-Affiliation
Veterinary Serum and Vaccine Research Institute, Abassia, Cairo
Email
-City
-Orcid
-MiddleName
-Affiliation
Veterinary Serum and Vaccine Research Institute, Abassia, Cairo
Email
-City
-Orcid
-Link
https://vmjg.journals.ekb.eg/article_368247.html
Detail API
https://vmjg.journals.ekb.eg/service?article_code=368247
Publication Title
Veterinary Medical Journal (Giza)
Publication Link
https://vmjg.journals.ekb.eg/
MainTitle
Preparation of Hemolysin for Detection of some Viral and Bacterial Antigens