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368246

Advanced studies on Mycoplasma mastitis in Egyptian cattle and buffaloes

Article

Last updated: 03 Jan 2025

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Tags

Bacteriology and bacterial diseases

Abstract

 Mycoplasma mastitis has been seen in Egypt which is charaterized by abnormal secretion followed by marked agalactiae which did not respond to treatment with antibiotics. Therefore, this study concerned with detection of mycoplasma mastitis in dairy cattle and buffaloes in six governorates using culture and molecular characterization. The highest prevalence was detected in cattle suffered from clinical mastitis at Menofia (73%), followed by Behera (50%), while Ismailia was the least (28.6%). In the buffaloes isolation was from Menofia (100%) and Behera (60%). The isolation rate from subclinical mastitis in cattle ranged from (0- 12.4%) and from buffaloes (0-54.5%). One hundred and twelve out of 151 isolates were identified as M. bovis, while only seven strains were M. bovirhinis and six M. arginini. On the other hand, 26 isolates were Acholeplasma species. The polymerase chain reaction (PCR) technique was used for the detection of M. bovis variable surface protein A gene (Vsp A gene) in dairy cattle and buffaloes suffered from mastitis. All positive samples by culture were positive by PCR. Sequencing of 16S rRNA gene of M. arginini isolate gave 99% homology with the reference strain (G 230). Comparing of M. bovis isolates (Vsp gene) with the reference strain (PG 45) cleared the identity of 96-98%, while the identity was 94-100% when compared with the Italian M. bovis field strain (Sar 1).  

DOI

10.21608/vmjg.2011.368246

Authors

First Name

Eissa

Last Name

I

MiddleName

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Affiliation

Mycoplasma Dept., Animal Health Research Institute Cairo, Egypt

Email

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City

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Orcid

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First Name

Hassan

Last Name

M

MiddleName

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Affiliation

Mycoplasma Dept., Animal Health Research Institute Cairo, Egypt

Email

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City

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Orcid

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First Name

Hashem

Last Name

H

MiddleName

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Affiliation

Mycoplasma Dept., Animal Health Research Institute Cairo, Egypt

Email

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City

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Orcid

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First Name

Abd El- Aziz

Last Name

E

MiddleName

-

Affiliation

Mycoplasma Dept., Animal Health Research Institute Cairo, Egypt

Email

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City

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Orcid

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First Name

Darder

Last Name

A

MiddleName

-

Affiliation

Mycoplasma Dept., Animal Health Research Institute Cairo, Egypt

Email

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City

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Orcid

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Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

197

Page End

209

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 197-209.pdf

PDF . 4.6MB

Link

https://vmjg.journals.ekb.eg/article_368246.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368246

Order

368,246

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Advanced studies on Mycoplasma mastitis in Egyptian cattle and buffaloes

Details

Type

Article

Created At

23 Dec 2024