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368236

First Molecular Detection and characterization of Borrelia burgdorferi sensu lato (Lyme disease spirochete) in Egyptian rodents

Article

Last updated: 23 Dec 2024

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Tags

Biotechnology/ molecular biology

Abstract

This study was intended to molecularly detect and characterize Borrelia burgdorferi senau lato in Egyptian rodents as an initial step in reservoir competence studies. A total of 30 rodents (26 Rattus norvegicus and 4 Rattus rattus) were caught at different areas in Giza Governorate, Egypt. EDTA-whole blood was collected and tested for the presence of spirochetal DNA using ospA targeting-PCR, and specific amplicons were then subjected to bidirectional sequencing. Positive amplification of 307-bp fragment was detected in 5 (19.2%) R. norvegicus (brown or Norway rat), while no borrelial DNA was evident in R. rattus (black rat). DNA sequence of the detected strain, designated as Borrelia burgdorferi Ghafar, was deposited in the GenBank under accession no. FJ968724. Sequence and phylogenetic analysis showed that Ghafar strain belongs to B. burgdorferi sensu stricto (99.6%- 100% similarity) and clearly distinguishable from other B. burgdorferi sensu lato genospecies. These results provide the first molecular report addressing existence of B. burgdorferi sensu stricto in Rattus norvegicus in the country, suggesting that this murine host may act as a reservoir for Lyme disease agent.

DOI

10.21608/vmjg.2011.368236

Keywords

Borrelia burgdorferi sensu lato, Rodents, Rattus norvegicus, Rattus rattus, PCR, Molecular characterization, Egypt

Authors

First Name

M

Last Name

Ghafar

MiddleName

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Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Egypt. Department of Biotechnology, College of Science, Taif University, KSA.

Email

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City

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Orcid

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First Name

A

Last Name

Saleh

MiddleName

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Affiliation

Department of Plant Protection, King Saud University, KSA.

Email

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City

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Orcid

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First Name

A

Last Name

Badawi

MiddleName

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Affiliation

Biochemistry Division, National Organization for Drug Control and Research, Egypt.

Email

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City

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Orcid

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First Name

A

Last Name

Abd El-Aty

MiddleName

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Affiliation

Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Egypt.

Email

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City

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Orcid

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Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

117

Page End

128

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 117-128.pdf

PDF . 2.0MB

Link

https://vmjg.journals.ekb.eg/article_368236.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368236

Order

368,236

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

First Molecular Detection and characterization of Borrelia burgdorferi sensu lato (Lyme disease spirochete) in Egyptian rodents

Details

Type

Article

Created At

23 Dec 2024