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368235

Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals

Article

Last updated: 03 Jan 2025

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-

Tags

Immunology / vaccine / diagnosis

Abstract

 Monospecific antisera against Paneurella multocida strains that belonged to capralar and somatic serogroups A:1, A:3 and A:4, Avibacterium paragallinarum serovars A and C and Newcastle disease virus, were prepared in three separate rabbit groups for each using reference bacterial and viral cultures emulsified in Freund's adjuvant. Antisera of P. multocida were qualitatively and quantitatively evaluated by serological tests using their homologous and heterologous antigens (cross matching). Sera of immunized rabbits showed a reasonable titre of P. multocida capsular group (A) antibodies by passive haemagglutination test and to somatic serotype (A) 1, 3 and 4, antibodies by tube Aghaination and agar gel diffusion tests. Serovars A and C antibodies of Avibacterium paragallinarum were detected in sera of the immunized rabbits by plate agglutination test. The main titre of 8.5 log2 haemagglutination inhibitory antibodies against Newcastle disease virus was achieved in sera of immunized rabbit which received 3 successive inoculation of ND LaSota strain. Conclusively, the attained results proved that the prepared mono-specific antisera could be used as a highly reliable guide in the serological identity tests of the challenge and vaccinal strains of fowl cholera, infectious coryza and Newcastle disease.  

DOI

10.21608/vmjg.2011.368235

Authors

First Name

Ghada

Last Name

El-Sadek

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

Amal

Last Name

El-Sawah

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

Hanaa

Last Name

Mostafa

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

Hanan

Last Name

Mahmoud

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

El-Safty

Last Name

M

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

Elham

Last Name

El-Ebiary

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

First Name

Abdel Baky

Last Name

H

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo

Email

-

City

-

Orcid

-

Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

109

Page End

116

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 109-116.pdf

PDF . 1.2MB

Link

https://vmjg.journals.ekb.eg/article_368235.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368235

Order

368,235

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals

Details

Type

Article

Created At

23 Dec 2024