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368230

Tissue culture propagation of highly pathogenic avian influenza H5N1 virus

Article

Last updated: 03 Jan 2025

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Tags

Virology and viral diseases

Abstract

   Avian influenza virus usually refers to influenza A viruses found chiefly in birds, but infections can occur in humans. The risk is generally low to most people, because the viruses do not usually infect humans. However, confirmed cases of human infection have been reported since 1997 by H5N1 subtype. In the current study, a highly pathogenic avian influenza subtype HSNI was isolated and confirmed by PCR and sequencing. Sequence analysis revealed a high degree of identity with other published sequences of H5N1 isolates from Egypt. The multiple basic amino acid sequence of the isolated virus was similar to the highly pathogenic strains of H5N1 (G-E/R-R- R/K-K-R.) and to other viruses isolated in Egypt in the previous years specially in the presence of arginine amino acid in the cleavage site motive of the hemagglutinin protein.  The isolated virus which designed as A/chicken/1000CLEVB/2010 was propagated on chicken embryo fibroblast tissue culture. The virus titer was rapidly increasing from 4.8 log10 /ml in the 1st passage to 8.5 log10/ml by the 3rd one. The HA properties of the virus was regained by treatment with 0.5µg of trypsin/10 min which open the gates for the production of inactivated tissue culture propagated vaccine.

DOI

10.21608/vmjg.2011.368230

Authors

First Name

Soliman

Last Name

A

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

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City

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Orcid

-

First Name

Afaf

Last Name

Khedr

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

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City

-

Orcid

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First Name

Nermeen

Last Name

Elsaid

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

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City

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Orcid

-

First Name

Abdel Baky

Last Name

H

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

-

City

-

Orcid

-

First Name

Elham

Last Name

Ebiary

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

-

City

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Orcid

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Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

47

Page End

63

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 47-63.pdf

PDF . 2.8MB

Link

https://vmjg.journals.ekb.eg/article_368230.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368230

Order

368,230

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Tissue culture propagation of highly pathogenic avian influenza H5N1 virus

Details

Type

Article

Created At

23 Dec 2024