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368228

Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test

Article

Last updated: 03 Jan 2025

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-

Tags

Virology and viral diseases

Abstract

     Foot-and-mouth disease virus (FMDV) serotypes (0/1/3/93) and (A/Egy/2009), grown on BHK-21 clone 13 monolayer cell line, inactivated with Binary Ethylenimine (BEI) and adjuvanted with 30% Alhydragel [AL(OH)3] were used for preparation of bivalent gel adjuvant vaccine currently used in Egypt 146s was measured using sucrose density gradient ultracentrifugation and vaccine dose was estimated as 4.2 µg of 146s viral proteins for each vaccine serotypes. Evaluation and testing of FMD vaccine for safety, sterility and potency were carried out. Cattle protective dose 50% (PD50) was determined. The specific developed neutralizing antibody responses against different doses of bivalent vaccine in correlation with the challenge exposure test for serotype (01/3/93) virus that was done 21 days post vaccination were undertaken. The obtained potency of a vaccine with an overall 50% cattle protective dose (PD50) value was 9.99 (PD50) for serotype (O/1/3/93).

DOI

10.21608/vmjg.2011.368228

Authors

First Name

Bakr

Last Name

A

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research institute, Abbassia, Cairo.

Email

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City

-

Orcid

-

First Name

Saad

Last Name

A

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

Barghouth

Last Name

M

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

Ibrahim

Last Name

M

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

Abd El Aty

Last Name

M

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

Aly

Last Name

M

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

First Name

Farag

Last Name

Α

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research institute, Abbassia, Cairo.

Email

-

City

-

Orcid

-

Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

27

Page End

37

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 27-37.pdf

PDF . 1.8MB

Link

https://vmjg.journals.ekb.eg/article_368228.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368228

Order

368,228

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test

Details

Type

Article

Created At

23 Dec 2024