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368297

Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Anim

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

 Two models of ELISA were developed for serological detection of Bovine Ephemeral fever (BEF) antibodies in the sera of vaccinated Buffaloes in comparison with serum neutralization test. Blocking ELISA (B. ELISA) was developed and standardized for detection of BEF antibodies. In this test, the binding capacity of rabbit BEF hyper immune sera to BEFV antigen was blocked in the presence of positive BEF vaccinated Buffaloes sera.       The sensitivity of both ELISA models was compared with serum neutralization test (SNT) using a total of 160 serum samples obtained from vaccinated Buffaloes where it was found that the sensitivity and specificity of B- ELISA and I-ELISA were (98.4% & not estimated) and (100% & 100%), respectively. These finding suggested that the both ELISA models were considered an inexpensive, rapid and sensitive serological methods that would be suitable for measuring of BEFV antibodies in vaccinated Buffaloes.

DOI

10.21608/vmjg.2011.368297

Authors

First Name

Barghouth

Last Name

M

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo

Email

-

City

-

Orcid

-

First Name

Hanna

Last Name

Mostafa

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo

Email

-

City

-

Orcid

-

First Name

Ismail

Last Name

I

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute; Abassia, Cairo

Email

-

City

-

Orcid

-

First Name

Nermeen

Last Name

Mahmoud

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo

Email

-

City

-

Orcid

-

First Name

Abd El-Bakey

Last Name

M

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo

Email

-

City

-

Orcid

-

First Name

Zaky

Last Name

F

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo

Email

-

City

-

Orcid

-

Volume

59

Article Issue

3

Related Issue

49193

Issue Date

2011-07-01

Receive Date

2024-07-20

Publish Date

2011-07-01

Page Start

19

Page End

25

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 59_Issue 3_Pages 19-25.pdf

PDF . 1.2MB

Link

https://vmjg.journals.ekb.eg/article_368297.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368297

Order

368,297

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Anim

Details

Type

Article

Created At

23 Dec 2024