The present study was planned to isolate and identify the local Equine herpes viral abortion (EHV-1). So, samples (Liver, lung, spleen, placenta and serum) of 5 aborted mares and their foeti were collected from a private stud in Egypt, with a history of recurrent abortion during (2005, 2006). Attempts for isolation of the causative agent were performed on chorioallantoic membrane of SPF embryonated chicken eggs and tissue culture (VERO cells). The highest titre was obtained from the liver of foeti which ranged from 8-9 log₁₀ EID₅₀ at 3^rd egg passage and 7-8 log₁₀ TCID₅₀ at 5^th tissue culture passage. These results were confirmed by different serological tests (AGPT, VN test, ELISA and IF) by using reference rabbit anti-EHV-1 sera. In addition, antibodies against EHV-1 were detected in mares sera by AGPT and ELISA.