In the present study, the effect of a mixture of some immunostimulant substances on the immune response of broiler chicks to bivalent AI-ND vaccine was determined. Four groups of chicks were used. Group 1 was vaccinated with one dose of AI-ND vaccine and simultaneously injected with the mixture of immunostimulant substances. Group 2 was vaccinated with one dose of the bivalent AI-ND vaccine. Group 3 was vaccinated with 2 doses of live ND vaccines (Hitchner then Lasota Strains) and group 4 was kept none vaccinated as control chicks. All birds were monitored Weekly for the humoral and cellular immune response then challenged with the virulent NDV at 35 days of age. HI test was used for titration of antibodies for both AIV and NDV, phagocytic activity, Nitric oxide; Lysozyme activity and total antioxidant in serum were used to determine the cellular immune response of the chicks. Protective immunity induced in the vaccinated groups varied. The injected immunostimulant mixture demonstrates its effect on the immune response to the bivalent AI-ND vaccine in group 1 with 100% protection against the challenge NDV. Whereas, 80 and 60 % protection were obtained in chicks vaccinated either with AI-ND vaccine (group 2) or live ND vaccines (group 3); respectively. The present study reports the effect of injection of some immunostimulant substances in augmentation of the immune response to the inactivated vaccinens.