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368053

Improved quality control Protocol for fowl pox virus vaccines based on the use of PCR and sequence analysis to detect REV and ALV as contaminants

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Virology and viral diseases

Abstract

In the present study, twenty one Fowl pox virus (FPV) vaccines were collected in the period between 2003-2005 and tested using the currently used and an improved quality control protocols. Results of currently used quality control protocol revealed negativity of all tested vaccines for the presence of contaminants and the results were satisfactory. Using PCR to detect Reticuloendotheliosis virus (REV) as contaminant in such vaccines revealed negative result except one suspected contaminated vaccine. Inoculation of this vaccine in egg, chicken embryo fibroblasts (CEFs), Specific pathogen free (SPF) chicks for three passages and testing of samples collected from inoculated host revealed positive amplification using REV Specific primers. Sequence analysis of the obtained amplification fragment for REV revealed its negativity and confirmed the non specific amplification of such primers which were previously published in several PCR studies for REV. Using avian leucosis virus (ALV) sets of primers to detect groups A, B, C, D and J in a PCR reaction revealed positive amplification of ALV fragment and confirming the contamination of tested vaccine with ALV. The study proposes the importance of using PCR followed by sequence analysis of the amplified product to confirm the contaminations found in the FPV vaccines.  

DOI

10.21608/vmjg.2011.368053

Authors

First Name

Radwan

Last Name

A

MiddleName

-

Affiliation

Central Laboratory of quality control for Evaluation of Veterinary Biologics, Abbassia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Hussein

Last Name

HA

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt, 12211

Email

-

City

-

Orcid

-

First Name

El-Ibiary

Last Name

A

MiddleName

-

Affiliation

Central Laboratory of quality control for Evaluation of Veterinary Biologics, Abbassia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

El-Safty

Last Name

D

MiddleName

-

Affiliation

Central Laboratory of quality control for Evaluation of Veterinary Biologics, Abbassia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

El-Sabagh

Last Name

M

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt, 12211

Email

-

City

-

Orcid

-

First Name

El-Deeb

Last Name

AH

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt, 12211

Email

-

City

-

Orcid

-

Volume

57

Article Issue

4

Related Issue

49190

Issue Date

2009-10-01

Receive Date

2024-07-18

Publish Date

2011-10-01

Page Start

617

Page End

629

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_368053.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=368053

Order

368,053

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Improved quality control Protocol for fowl pox virus vaccines based on the use of PCR and sequence analysis to detect REV and ALV as contaminants

Details

Type

Article

Created At

23 Dec 2024