One hundred and ten milk samples were collected from 35 dairy cows with recurrent mastitis and 75 apparently healthy ones. The field application of California Mastitis Test (CMT) on the apparently healthy cows indicated 60% (45 out of 75 cows) with sub-clinical mastitis. The estimation of SCC recorded 31.8% (35 samples) above 1x10⁶ cell/ml, 40.9% (45 samples) were ranged between 4x10⁵ to 1x10⁶ cell/ml and 27.3% (30 samples) were less than 4x10⁵cell/ml. The bacteriological examination revealed 10% facultative anaerobic bacteria from normal milk samples, while both facultative and obligatory anaerobic bacteria were 62.2% from sub-clinical and 100% from clinical mastitic milk samples. The highly effective antibiotics on the isolated bacteria in the laboratory were  florofenicol, tetradelta, gentamicin, amoxicillin-clavulanate,refampicin and metronidazol.