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365334

Bacteriological Examination and Diagnostic Performance of Some Serological Tests Used In Diagnosis of Small Ruminant Brucellosis

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Last updated: 03 Jan 2025

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Abstract

A total of 249 serum samples was collected from 120 ewes and 129 goats from the Nile delta Governorates with no history of vaccination against brucellosis and with sporadic cases of late-term abortions. Collected serum samples were serologically examined for brucellosis using the screening tests; buffered acidified plate antigen (BAPA), modified Rose-Bengal (mRBT) and indirect enzyme linked immunosorbent assay (iIELISA). Also the supplementary micro-agglutination (MAT), EDTA modified micro-agglutination tests, as well as the confirmatory complement fixation (CFT) and Rivanol- precipitation plate agglutination tests (Riv. T) were used. The aim was to evaluate the diagnostic performance of these tests as regards to relative sensitivity/specificity, area under the receiver operating characteristic curve (AUC), diagnostic odds ratio (DOR) and kappa agreement (k) with complement fixation test (gold standard). Good diagnostic significance was proved with BAPA, mRBT and iELISA in the form of high relative sensitivities on the expense of relative specificities, substantial agreement with CFT, high DORs and better performance based on ROCs and AUCs. Questionable diagnostic results were recorded by MAT render it unreliable for diagnosis of small ruminant brucellosis. The diagnostic performance of Riv.T didn't fulfil the requirements for the confirmatory test particularly in sheep where the test recorded the lowest relative sensitivity and specificity of 73% and fair agreement (0.224) with the CFT, lowest DOR (7.35)and AUC (0.8). These results subsequently reclassified the test as a supplementary rather than a confirmatory test. Bacteriological examination of samples from small ruminants resulted in the recovery of 9 isolates of Brucella melitensis biovar 3.

DOI

10.21608/vmjg.2016.365334

Keywords

Bacteria, Brucellosis — serological — small ruminants

Authors

First Name

Ola

Last Name

Abdel-Mortada

MiddleName

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Affiliation

Microbiology Department, Faculty of Agriculture, Ain Shams University

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Orcid

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First Name

Nour

Last Name

Abdel-Hamid

MiddleName

-

Affiliation

Department of Brucellosis Research Animal Health Research Institute, Nadi EL-Seid Street, Dokki, Giza 12618, Egypt

Email

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City

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Orcid

-

First Name

Hemmat

Last Name

Abdel hady

MiddleName

-

Affiliation

Microbiology Department, Faculty of Agriculture, Ain Shams University

Email

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City

-

Orcid

-

First Name

Rania

Last Name

Farouk

MiddleName

-

Affiliation

Microbiology Department, Faculty of Agriculture, Ain Shams University

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-

City

-

Orcid

-

Volume

62

Article Issue

4

Related Issue

47599

Issue Date

2016-10-01

Receive Date

2024-07-06

Publish Date

2016-10-01

Page Start

11

Page End

21

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 62_Issue 4_Pages 11-21.pdf

PDF . 5.0MB

Link

https://vmjg.journals.ekb.eg/article_365334.html

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https://vmjg.journals.ekb.eg/service?article_code=365334

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365,334

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Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Bacteriological Examination and Diagnostic Performance of Some Serological Tests Used In Diagnosis of Small Ruminant Brucellosis

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Article

Created At

23 Dec 2024