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363158

Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

This study aimed to compare the efficiency of dimethyl-formamide (DMF), glycerol and their combination on freezability of Arabian stallion spermatozoa. Fourty semen samples were collected from four Arabian stallions, diluted with INRA-82 supplemented with 5% glycerol, 5% DMF or 2.5% glycerol+2.5% DME and subjected to cryopreservation. The results revealed the superiority of DMF vs. glycerol in terms of post-thaw motility (56.25 ± 0.89 vs. 46.50 ± 1.14%, respectively), viability index (167.75 ± 3.49 vs. 135.50 ± 4.49, respectively), survival rate (74.58 ± 1.03 vs. 61.59 ± 0.38%, respectively) and sperm acrosome integrity (78.83 ± 0.74 vs. 75.95 ± 0.74%, respectively). In conclusion, The protocols of Arabian stallion sperm cryopreservation could be enhanced using DMF as a cryoprotectant (CPA) used with INRA-82 extender.

DOI

10.21608/vmjg.2016.363158

Keywords

Arabian stallion- glycerol- dimethyl-formamide- cryoprotectant, freezing

Authors

First Name

Aya

Last Name

Fadl

MiddleName

-

Affiliation

Theriogenology Dept., Faculty of veterinary medicine, Cairo university

Email

-

City

-

Orcid

-

First Name

El-Badry

Last Name

A

MiddleName

-

Affiliation

Artificial insemination and embryo transfer Dept., Anim. Reprod . Res. Instit.

Email

-

City

-

Orcid

-

First Name

Abou Ahmad

Last Name

M

MiddleName

-

Affiliation

Theriogenology Dept., Faculty of veterinary medicine, Cairo university

Email

-

City

-

Orcid

-

First Name

Ghallab

Last Name

M

MiddleName

-

Affiliation

Theriogenology Dept., Faculty of veterinary medicine, Cairo university

Email

-

City

-

Orcid

-

Volume

62

Article Issue

2

Related Issue

47597

Issue Date

2016-04-01

Receive Date

2024-07-01

Publish Date

2016-04-01

Page Start

59

Page End

63

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 62_Issue 2_Pages 59-63.pdf

PDF . 2.4MB

Link

https://vmjg.journals.ekb.eg/article_363158.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=363158

Order

363,158

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa

Details

Type

Article

Created At

23 Dec 2024