Abattoirs considered as sources of valuable information on the incidence of animal diseases and conditions, some of which may be zoonotic, as bovine tuberculosis which still remains a major health problem. Effort to improve food safety is going on all the time, regarding to the strategy of slaughtering cattle. Tuberculosis (TB) is an important zoonotic disease. In this study, a total of 175 (115 samples of prescapular, prefemoral, axillary and poplitial lymph nodes and 20 liver and hepatic lymph nodes samples ,20 kidney and renal lymph nodes samples and 20 spleen samples) were collected from some Egyptian abattoirs in Aswan and Red Sea governorates from imported slaughtered animals. The obtained results showed that, the total isolates, all of them were from internal organs and lymph nodes, and all of them were identified and the results of microscopical examination of different organs collected from tuberculin positive animals by Z.N stain revealed that 115 out of 175 examined samples showed acid fast bacilli. The lymph nodes had significantly higher number of positive results concerning to culture technique which revealed the isolation of mycobacteria from 137 positive samples out of 175 examined samples from the selected governorates in percentage of isolation reached to 65.7 % and 78.3 % respectively. The results of real time PCR technique revealed that, all of tested tissue samples of 175 tuberculin positive animals were positive with percentage (100%). These results proved the accuracy of the judgment policy of the Egyptian organization which able to eliminate all the positive cases of bovine tuberculosis in some examined governorates.