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355072

Influence of linoleic acid supplementation on the quality of frozen-thawed ram semen

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Theriogenology / reproductive technology

Abstract

The present study was conducted to evaluate the effect of linoleic acid (LA) supplementation in the freezing diluent on the quality of frozen-thawed ram semen. Semen was collected from five mature fat tailed rams once weekly using the conventional A.V. of rams. Semen samples were processed as a pool, divided into 5 aliquots, each aliquot was diluted with egg yolk-Tris-glycerol diluent and three concentrations (50, 100 and 150 μM) of LA were used. Diluted semen was then cooled, equilibrated and subjected to cryopreservation. Evaluation of post-thaw semen quality included progressive sperm motility, alive and abnormal sperm percentages, functional membrane integrity (HOST) and acrosome integrity. Results showed that the percentages of alive sperm (51.4 vs 40.2%; P< 0.05) and HOST (53.2 vs 39.6%; P< 0.05) were significantly increased only at concentration of 50 μM of LA compared to control, respectively. Also, LA (50 μM) decreased significantly (P<0.05) the percentage of abnormal sperm compared to control with ethanol (20.6 vs 29%). Regarding the intact acrosome percentage, only the 150 μM LA decreased significantly (P<0.05) the intact acrosome (75 vs 80.4%) compared to control. The effects of the other concentrations on the other post-thaw semen quality parameters were non significant. It could be concluded that, addition of LA (50 μM) to Tris based diluent improved the post-thaw quality of ram semen in terms of sperm membrane integrity and sperm morphology.

DOI

10.21608/vmjg.2024.355072

Keywords

Semen cryopreservation, n-6 fatty acids, linoleic acid and ram

Authors

First Name

H.

Last Name

Elsherbiny

MiddleName

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Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Ghallab

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Abou-Ahmed

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University; Giza, Egypt

Email

-

City

-

Orcid

-

Volume

63

Article Issue

2

Related Issue

44993

Issue Date

2017-04-01

Receive Date

2024-05-19

Publish Date

2024-05-19

Page Start

21

Page End

25

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 63_Issue 2_Pages 21-25.pdf

PDF . 3.1MB

Link

https://vmjg.journals.ekb.eg/article_355072.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=355072

Order

355,072

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Influence of linoleic acid supplementation on the quality of frozen-thawed ram semen

Details

Type

Article

Created At

23 Dec 2024