The study aims to evaluate the effect of L-carnitine supplementation on some blood parameters and reproductive performance of buffaloes at late pregnancy period. A number of twenty-one buffaloes (3-4 lactations) were chosen to carry out the experimental work. The animals were divided into three groups (7 buffaloes each):- Group (GO) served as (control) don't receive any supplementation. Group (G1) supplemented with 5.0 gm L- carnitine /h/day. Group (G2) supplemented with 7.0 gm L-carnitine h/day. Both groups (G1 and G2) were supplemented with L-carnitine before parturition with two months and continued three months after parturition. During the experimental period, close observation was undertaken at the late pregnancy, one day before delivery buffaloes were kept in the delivery units until time of calving. Blood samples were collected weekly via the jugular vein from each buffalo through the experimental period and used for assay of plasma Glucose, total protein, blood urea nitrogen, creatine, creatinine, ALT, AST, Total lipids, cholesterol, triglycerides, progesterone (P4) and estradiol 178 hormones. All animals were observed for heat detection. The number of days open (DO) was recorded for each dam as the period elapsed from parturition to next conception. Number of services per conception (NSPC) was recorded as the total number of services divided by the total number of animals conceived. Results indicated that buffaloes supplemented with different levels of L- carnitine had an improved blood parameters in postpartum period except triglycerides. Although, concentrations of total protein, creatine and ALT activity in pre-partum period were not significantly (P>0.05) affected by L-camitine supplementation. L-carnitine supplementation enhanced oestrous sings especially mounting, response to finger massage, vaginal mucus discharge and intensity of oestrous activity. For reproductive performance, significant differences were recorded among the groups in studied parameters such as NSPC, CI, mean period elapsed from calving until placenta drop, the interval required for each of pregnant uterus to return intra-pelvic, postpartum cervical closure and uterine horns symmetry and ovulation time. It can be concluded that, L-carnitine can be used for buffalo cows during late pregnancy and early lactation to enhance their reproductive performance.