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268164

Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin

Article

Last updated: 23 Dec 2024

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Tags

Anatomy
Histology

Abstract

The objective of the current study is to evaluate using of platelet-rich fibrin (PRF) or adipose-derived stem cells (ADSCs) for the therapy of rabbit ear cartilage defects. Nine adult New Zealand white rabbits were divided into three groups. The rabbit ears developed two defects in the cartilage. Phosphate-buffered saline (PBS) was injected sub- perichondrially into Group I, adipose-derived MSCs (ADMSCs) were injected into Group II, and platelet-rich fibrin (PRF) was injected into Group III in the defective ear. After 4 weeks, the auricular defect was examined morphologically, histologically, and immunohistochemically. In addition, the gene expression of the collagen type II (Col II) and aggrecan. Anatomically, the auricles of all treatments seemed to be fully healed with smooth surfaces. Histopathologically, a small region of immature cartilage was seen in the control positive group's defects. While the group treated with PRF and ADSCs demonstrated the normal characteristics of new cartilage development, including mature chondrocytes within their lacunae and rich extracellular matrix (ECM). The PRF-treated group responded to Masson's trichrome and orcein stains, while produced a faint staining in the case of the control positive and ADMSC groups. Immunohistochemically, there was a strong positive S100 expression in ADSCs and PRF but mild reaction in the control group. The expression of relative col II and aggrecan was substantially highest in PRF (± 0.72 and ± 0.57, respectively). While, Control positive and ADMSC groups recorded (± 0.41 : ± 0.21, ± 0.6 : ± 0.44) respectively. The PRF had the highest rate of proliferation and chondrogenic potential when compared to the ADMSCs and control groups.

DOI

10.21608/vmjg.2022.268164

Keywords

Auricular cartilage defects, Adipose stem cells, Platelet rich fibrin, Rabbit

Authors

First Name

Taghreed

Last Name

Hassan

MiddleName

-

Affiliation

Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Maher

MiddleName

-

Affiliation

Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Email

mohamed.om32@cu.edu.eg

City

-

Orcid

-

First Name

Amr

Last Name

El Karmoty

MiddleName

F.

Affiliation

Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Email

amr.elkarmoty@yahoo.com

City

-

Orcid

-

First Name

Zainab

Last Name

Ahmed

MiddleName

S. O.

Affiliation

Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Email

zainab.sabry@cu.edu.eg

City

-

Orcid

-

First Name

Marwa

Last Name

Ibrahim

MiddleName

A.

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Hamdy

Last Name

Rizk

MiddleName

-

Affiliation

Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Email

hamdyrezk81@cu.edu.eg

City

-

Orcid

-

First Name

Ayman

Last Name

Tolba

MiddleName

-

Affiliation

Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Email

-

City

-

Orcid

-

Volume

68

Article Issue

1

Related Issue

39232

Issue Date

2022-12-01

Receive Date

2022-11-02

Publish Date

2022-12-01

Page Start

64

Page End

79

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 68_Issue 1_Pages 64-79.pdf

PDF . 1.1MB

Link

https://vmjg.journals.ekb.eg/article_268164.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=268164

Order

5

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin

Details

Type

Article

Created At

23 Dec 2024