Marine  biosurfactants  are  surface  active  agents  of  marine  origin  either produced by planktonic microorganisms of marine environment whether sea water or soil or produced by associated microorganisms with marine organisms.  In  general,  biosurfactants  are  classified  according  to categories of; Molecular structure, molecular weight and microbial origin. Mineral  salts  medium  “MSM"  supplemented  with  a  hydrophobic compounds  is  mainly  used  for  biosurfactant  production  enhancement. Then,  screening  of  biosurfactant  activity  is  operated  and  it  is  based  on qualitative tests which gave a true general indication of biosurfactant type and  potency  and  quantitative  test  which  gave  a  precise  reading  of  the quantity of biosurfactant and its type. Qualitative tests are mainly; blood hemolysis,  oil  spreading  test,  Emulsification  index  test  “E24",  Drop collapse  test  and  CTAB  assay  which  detects  anionic  biosurfactants,  no single method is suffiecient for detection of biosurfactant or bioemulsifier activity. While, a quantitative test gave us a precise reading of the surface tension, more than one qualitative screening method is required to prove a  biosurfactant  activity.  Biosurfactants  have  been  discussed  by  many studies  on  approximately  all  life  aspect  of  food,  agricultural, pharmaceutical, cosmetic, medical, laundry industry, petroleum industry, and  environmental  applications.  They  have  proved  efficiency  which  is similar  or  even  better  than  synthetic  surfactants.  However,  they  are prefereed over synthetic ones in terms of biodegradability, environmental compatibility  and  in  case  of  marine  produced  biosurfactants  halophilic resistance  which  may  be  beneficial  from  economic  point  of  view.  So, many studies have focused on marine biosurfactants either for isolation of new  potent  strains,  their  identification  using  16s  rRNA  or  for  their potentials' applications utilizing their unique properties.