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369050

An Approach for Developing a Method for Biosurfactant Activity Detection

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Microbiology and biotechnology

Abstract

Detection  of  biosurfactant  activity  have  been  proposed  Previously  by several studies either quantitatively or qualitatively. Quantitative methods give precise measurement of the surface tension using accurate devices while  qualitative  measurements  are  considered  screening  methods  for biosurfactant activity detection with lower precision. Due to the high cost of the direct measurement devices and in some cases the unavailability of devices,  qualitative  methods  remain  the  most  common  used  tests  for screening of the surface-active properties. The need for an accurate, cheap method was the reason for this trial of developing a photometric method for  a  rapid,  accurate  biosurfactant  activity  detection.  “α-naphthol- butanol"  method  is  based  on  measuring  the  photometric  activity  of  a sparingly soluble compound “α-naphthol" at its λmax. Formed emulsion of oily  phase  “containing  α-naphthol"  and  aqueous  phase  is  based  on  the surfactant  concentration  and  the  surface  tension  of  the  solution  being tested. This method passed the first and second confirmatory tests but, in the  third  trial  it  failed.  Knowing  the  drawbacks  of  this  method  we  can manage to develop a promising modification.  

DOI

10.21608/rpbs.2024.242587.1249

Keywords

Null hypothesis, surface activity detection, α-naphthol butanol, new method development

Authors

First Name

Mahmoud

Last Name

AbdelFatah

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

Email

ph.mahmoud.abdelfatah@gmail.com

City

-

Orcid

-

First Name

Ali

Last Name

Abdelrahman

MiddleName

Abdellah

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, , Suez Canal University, Ismailia, Egypt.

Email

dr_ali_abdellah@yahoo.com

City

-

Orcid

-

First Name

Samar

Last Name

Solyman

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

Email

samar.mansour@su.edu.eg

City

-

Orcid

-

Volume

8

Article Issue

2

Related Issue

47568

Issue Date

2024-05-01

Receive Date

2023-10-14

Publish Date

2024-05-01

Page Start

106

Page End

114

Print ISSN

2536-9857

Online ISSN

2535-2091

Link

https://rpbs.journals.ekb.eg/article_369050.html

Detail API

https://rpbs.journals.ekb.eg/service?article_code=369050

Order

369,050

Type

Mini-reviews

Type Code

534

Publication Type

Journal

Publication Title

Records of Pharmaceutical and Biomedical Sciences

Publication Link

https://rpbs.journals.ekb.eg/

MainTitle

An Approach for Developing a Method for Biosurfactant Activity Detection

Details

Type

Article

Created At

23 Dec 2024