Abstract: In recent years, increased scrutiny of food products and dietary supplements has highlighted the need for precise and accurate analytical methods to ensure component safety from production to consumption. Thus, the present study was aimed to develop a rapid, sensitive and green liquid chromatographic method for measuring ascorbic acid (ASA). The experiment was carried out by using a simplest HPLC, employing an isocratic elution setup, and lowering the amount of organic modifier, with a ZORBAX Eclipse C18 column (4.6 x 150 mm, 5 µm) at room temperature. The mobile phase consisted of methanol and phosphate buffer, adjusted to pH 3 ± 0.1 in a 10:90 (v/v) ratio. Ultraviolet detection at 254.00 nm was used for monitoring the eluent. Accuracy was found to have an RSD of 1.255%. Intra- and inter-day precision were both under 2.0% RSD, with values of 1.494% and 1.236%, respectively, and recovery ranged between 100-102%. The limit of detection was 1.080 µg/mL, and the limit of quantitation was 3.260 µg/mL. The developed method was effectively applied to the ASA dosage form. The evaluation of the developed method's greenness yielded a high rating of 0.89, demonstrating its practical reliability and effectiveness.
Keywords: Ascorbic Acid, High-Performance Liquid Chromatography, Dietary Supplements.