Pseudomonas aeruginosa (P. aeruginosa) is considered as the foremost cause of hospital -acquired infections due to its innate and plasmid mediated resistance to multiple antibiotics making it a multi-drug resistant (MDR) pathogen. In our cross–sectional study, one hundred and twenty-six (126) non-duplicate clinical P. aeruginosa isolates were recovered from 450 clinical specimens from burn units in Ismailia Hospitals. The antibiotic sensitivity of strong and moderate biofilm producers isolates was investigated using the disc diffusion method. The isolated bacteria were tested for their ability to form biofilm using a microtiter plate assay. The MPA detected 80% (95 /126) isolates as biofilm producers, 18% (22/126) were strong biofilm producers, 34% (43/126) were moderate biofilm producers, 28% (35/126) were weak biofilm producers and 20% (31/126) non biofilm producers. Susceptibility pattern analysis of biofilm forming P. aeruginosa isolates (95) detected that 60% (68/ 95) were multi-drug resistant isolates (MDR). Resistance to all used antibiotics and multidrug resistance was higher among biofilm producing than non-biofilm producing strains, but the difference was statistically non-significant. The present study confirmed that antimicrobial resistance was more prominent in biofilm-producing P. aeruginosa than in non-biofilm-producers.