Azithromycin (AZT) belongs to a family of macrolides with bacteriostatic activity against many Gram-positive and Gram-negative bacteria. The aforementioned drugs are of great importance due to the high rate of resistance to other antibiotics. Therefore, numerous research studies have been conducted to identify azithromycin and its metabolites as they contribute to the drug's biological activity. Liquid chromatography, gas chromatography, and other separation techniques have been used to develop, adapt, and implement analytical techniques for drug analysis. This brief overview aims to highlight the analytical techniques and new techniques implemented for Azithromycin analysis.