Sponges (phylum Porifera) are sessile marine filter feeders that have developed efficient defense mechanisms against foreign attackers such as viruses, bacteria, or eukaryotic organisms. Marine sponges considered to be a great source of pharmacologically of pharmacologically-active chemicals from marine organisms. Higginsia sp. (Family Desmoxyidae) was collected from the Red Sea and possesses a great number of secondary metabolites. The present study showed that, the unsaponifiable matter of Higginsia sp. contains various compounds identified as hydrocarbons (23.07%), fatty alcohols (32.32%) as well as other oxygenated compounds (21.62%). While, the saponifiable matter contains various compounds identified as methyl and ethyl esters of saturated fatty acids (34.54%) in addition to unsaturated fatty acids (35.37%) while other compounds (30.09%) couldn't be identified. The identification of the compounds depends on the retention
time and mass spectrum.