A total of twenty-six diverse actinomycetes were obtained from various regions of the Mediterranean Sea in Alexandria, Egypt. These isolates underwent primary screening against multiple Gram-positive, Gram-negative, yeast, and fungal strains. In the secondary screening, nine isolates exhibited varying degrees of activity against the tested microorganisms. Among them, isolate A7 demonstrated potent antimicrobial properties and was chosen for further investigation. Through morphological, biochemical, microscopic, and molecular identification techniques, isolate A7 was identified as Streptomyces mutabilis. The study also explored the influence of nutritional and physical factors on the antimicrobial activity of the agent generated by isolate A7. The optimal conditions for antimicrobial activity were found to be a pH value of 6, an incubation temperature of 37°C, a NaCl concentration of 4 g/L, 1.5% starch concentration, 0.4% sodium nitrate concentration, and 2.5 g/L of K₂HPO₄.3H₂O. Additionally, 0.5 g/L of MgSO₄ was incorporated into the broth media. The incubation was performed in a shaking incubator for 7 days at 150 rpm. Ethyl acetate was used in combination with thin-layer chromatography to extract and purify the antimicrobial agent. Fraction No. 6 exhibited the highest activity against the tested microorganisms. The purified compound was identified as Phenol, 2-Methoxy-4-(2-Propenyl). It displayed antimicrobial activity against various microorganisms and demonstrated significant antiviral activity without any observed cytotoxic effects on the tested cell line.